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| 无机/金属纳米颗粒与木瓜蛋白酶组装的研究 | |
| 引用本文： | 王安明,宗璟,黄振龙,姚忠,沈树宝,薛建跃.无机/金属纳米颗粒与木瓜蛋白酶组装的研究[J].离子交换与吸附,2008,24(1):10-15. |
| 作者姓名： | 王安明 宗璟 黄振龙 姚忠 沈树宝 薛建跃 |
| 作者单位： | 1. 南京工业大学制药与生命科学学院,南京,210009;巢湖学院化学系,巢湖,238300 2. 南京工业大学制药与生命科学学院,南京,210009 3. 巢湖学院化学系,巢湖,238300 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，江苏省自然科学基金，安徽省高校青年教师科研项目 |
| 摘 要： | 运用氧化还原反应在二氧化硅表面沉积组装了银纳米粒子,并在该载体表面共价连接木瓜蛋白酶,试图组合超微载体与化学共价固定化酶的优点,进一步提高组装后的酶活性.初步研究了微球尺寸及银纳米粒子对组装酶活力影响.实验结果表明,在给酶量为33.3mg/g时,木瓜蛋白酶直接共价连接在二氧化硅表面时活力仅为402u/mg,而在组装有银的载体上时活力为536u/mg,催化活性提高30%左右.同时在高给酶量条件下,酶活力回收提高了60%左右,活力回收最大值达到了30.5%,明显优于戊二醛直接共价固定的文献报道值. |
| 关 键 词： | 二氧化硅 纳米粒子 木瓜蛋白酶 活力回收 |
| 文章编号： | 1001-5493(2008)01-0010-06 |
| 收稿时间： | 2007-01-19 |
| 修稿时间： | 2007年1月19日 |
STUDY ON CO-ASSEMBLY OF INORGANIC/METAL HYBRID NANOPARTICLES AND PAPAIN |
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| WANG Anming,ZONG Jing,HUANG Zhenglong,YAO Zhong,SHEN Shubao,XUE Jianyue.STUDY ON CO-ASSEMBLY OF INORGANIC/METAL HYBRID NANOPARTICLES AND PAPAIN[J].Ion Exchange and Adsorption,2008,24(1):10-15. | |
| Authors: | WANG Anming ZONG Jing HUANG Zhenglong YAO Zhong SHEN Shubao XUE Jianyue |
| Abstract: | Silver nanoparficles formed by oxidation-reduction were deposited on the silica spheres and papain was covalently linked to the spheres, which was intended to combine the advantages of ultra-structure support and covalent immobilization to improve the activity of assembled papain. Effects of silver nanoparticles and diameter of silica spheres on the activity of papain were studied. The results showed that, the activity of papain was 402u/mg when coupled to bare silica spheres, but to silica spheres with deposition of silver nanoparticles it was 536u/mg, an increase by about 30%. The activity recovery of papain also increased by about 60% and maximum reached 30.5%, which was obviously better than that literature value in covalent immobilization with glutaraldehyde. |
| Keywords: | Silica Nanoparticles Papain co-assembly |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |