Simple determination of forphenicinol in human plasma and erythrocytes by HPLC with native fluorescence detection |
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Authors: | M Kai K Tamura M Ohno Y Ohkura |
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Institution: | Faculty of Pharmaceutical Sciences, Kyushu University 62, Fukuoka, Japan. |
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Abstract: | A high performance liquid chromatographic method is described for monitoring forphenicinol, a possible therapeutic drug for cancer and muscular dystrophy, in human plasma and erythrocytes. Forphenicinol in the deproteinized samples was separated from interfering biogenic substances on an aminopropyl-bonded silica (Unicil NH2) column within 10 minutes with isocratic elution, and determined with fluorescence detection. The detection limits for forphenicinol in plasma and erythrocytes are 65 pmol (12.8 ng)/ml and 160 pmol (31.5 ng)/ml, respectively, corresponding to 2 pmol each in a 100 microliters injection volume. The method is very simple, and sensitive enough to permit the quantification of forphenicinol in the blood samples from man dosed with forphenicinol. |
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