A novel method of DNA transfection by laser microbeam cell surgery |
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| Authors: | M. Tsukakoshi S. Kurata Y. Nomiya Y. Ikawa T. Kasuya |
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| Affiliation: | (1) The Institute of Physical and Chemical Research, 351 Wako, Saitama, Japan;(2) Present address: Laboratory of Human Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan |
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| Abstract: | A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. |
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| Keywords: | 07.60 42.80 87 |
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