Asymmetric bioreduction of alkenes using ene-reductases YersER and KYE1 and effects of organic solvents |
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Authors: | Yanto Yanto Winkler Christoph K Lohr Stephanie Hall Mélanie Faber Kurt Bommarius Andreas S |
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Affiliation: | School of Chemical and Biomolecular Engineering, Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0363, United States. |
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Abstract: | Asymmetric trans-bioreduction of activated alkenes by KYE1 from Kluyveromyces lactis and Yers-ER from Yersinia bercovieri, two ene-reductases from the Old Yellow Enzyme family, showed a broad substrate spectrum with a moderate to excellent degree of stereoselectivity. Both substrate- and enzyme-based stereocontrols were observed to furnish opposite stereoisomeric products. The effects of organic solvents on enzyme activity and stereoselectivity were outlined in this study, where two-phase systems hexane and toluene are shown to sustain bioreduction efficiency even at high organic solvent content. |
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