A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells |
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作者姓名: | Tun Tang Yu-Qi Chen Bo-Shi Fu Zhi-Yong He Heng Xiao Fan Wu Jia-Qi Wang Shao-Ru Wang Xiang Zhou |
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作者单位: | College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China |
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基金项目: | supported by the National Basic Research Program of China (973 Program, Nos. 2012CB720600, 2012CB720603);the National Science Foundation of China (Nos. 91413109, 21202126);East Lake High-tech Zone 3551 Talents Scheme |
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摘 要: | In this study, a resorufin derivative RTP-1, which is a novel fluorescent ‘‘turn-on' probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 mmol/L, with a detection limit of 0.84 mmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory(DFT).Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine(100 mmol/L) for 30 min.
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关 键 词: | Resorufin Fluorescent probe Hydrazine Selectivity Cell imaging |
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