A fast, simple colorimetric determination of total urinary estrogen |
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Authors: | R. J. Laplante R. J. Thibert H. S. Asselstine |
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Affiliation: | 1. Department of Chemistry, University of Windsor, Windsor, Ontario, N9B 3P4, Canada;2. Department of Pathology, Metropolitan General Hospital, Windsor, Ontario, N8W 1L9, Canada |
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Abstract: | An improved method for the kinetic determination of creatinine on the Abbott ABA-100 is described. By simplex optimization of the picrate and hydroxide reaction concentration a 35% increase in reaction rate, with respect to the Abbott methodology, was obtained. The new method correlated more closely with the Technician AutoAnalyzer Method (N-30) than did the latter method. The percentage CV for the improved method for high and low quality control sera was 2.4 and 3.6%, respectively. The presence of protein in the standards was found to cause a decrease in the reaction rate with respect to aqueous standards. |
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