Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip |
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| Authors: | Hromada Louis P Nablo Brian J Kasianowicz John J Gaitan Michael A DeVoe Don L |
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| Affiliation: | University of Maryland, College Park, Department of Mechanical Engineering, College Park, Maryland 20742, USA. |
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| Abstract: | The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented. |
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