中药指纹图谱共有峰的自动识别

依托时间匹配、光谱信息辅助,利用自编的VB程序,建立并比较了时间匹配和时间匹配参考光谱信息两种识别中药指纹图谱共有峰的方法,并成功地应用于肉苁蓉的高效液相色谱-二极管阵列检测器(HPLC-DAD)法指纹图谱的建立.结果表明,只需要人为设置色谱峰大小下限、保留值相对误差上限和最大吸收波长差值上限三个参数,VB程序编制的指纹分析系统就能快速对所有样本进行共有峰识别和模糊数学处理,实现了无人工干预下的中药指纹图谱共有峰的自动识别,指纹图谱的建立过程客观,并且节省大量时间,其中参考光谱法识别结果更准确.

Automatic Identification of the Common Peaks in the Chromatographic Fingerprints of Traditional Chinese Medicines

Automatic identification of the common peaks in the chromatographic fingerprints of traditional Chinese medicines was established based on two methods of time matching and time-spectrum-matching identification which are based on the relative retention time and spectrum of each peak,respectively.Automatic identification was accomplished by the self-built program utilizing VB programming language.Two methods have been successfully applied in the chromatographic fingerprints of Cistanche salsa.It showed that i...