Immobilized horseradish peroxidase as a reusable catalyst for emulsion polymerization |
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Authors: | Naves Alliny F Carmona-Ribeiro Ana M Petri Denise F S |
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Institution: | Instituto de Química, Universidade de S?o Paulo, P.O. Box 26077, S?o Paulo SP, 05513-970, Brazil. |
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Abstract: | The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of in situ ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate (EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times. |
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