Investigations of the interaction between cuprous oxide nanoparticles and Staphylococcus aureus |
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| Authors: | ChengLing Shen YuanFang Li WenJing Qi and ChengZhi Huang |
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| Institution: | (1) Key Laboratory on Luminescence and Real-Time Analysis of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China;(2) College of Life Science, Southwest University, Chongqing, 400715, China;(3) College of Pharmaceutical Science, Southwest University, Chongqing, 400715, China |
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| Abstract: | In this study, cuprous oxide nanoparticles of 30–50 nm in size were prepared in the presence of cetyltrimethylammonium bromide
(CTAB). By taking Staphylococcus aureus (S.a), which always causes a variety of suppurative infections and toxinoses in humans, as a model bioparticle, the negative bioeffect
of nano-Cu2O on S.a cells was evaluated, and minimal inhibitory concentration (MIC) was determined by imitating the MIC of antibiotics.
Cellularity and bactericidal effect were measured by flow cytometry (FCM), dark field light scattering imaging and SEM photography.
The results showed that nano-Cu2O particles may, by absorbing on the cell surface, impair the cell wall, damage the cell membrane, and finally increase permeability
of the cell membrane, thus leading to a decrease in the viability of bacteria in the nano-Cu2O solution.
Supported by the National Natural Science Foundation of China (Grant Nos. 30570465 & 20425517) |
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| Keywords: | cuprous oxide nanoparticles Staphylococcus aureus minimal inhibitory concentration (MIC) flow cytometry (FCM) |
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