Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution |
| |
Authors: | Tafelmeyer Petra Johnsson Nils Johnsson Kai |
| |
Institution: | Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. |
| |
Abstract: | Split-protein sensors have become an important tool for the analysis of protein-protein interactions in living cells. We present here a combinatorial method for the generation of new split-protein sensors and demonstrate its application toward the (beta/alpha)(8)-barrel enzyme N-(5'-phosphoribosyl)-anthranilate isomerase Trp1p from Saccharomyces cerevisiae. The generated split-Trp protein sensors allow for the detection of protein-protein interactions in the cytosol as well as the membrane by enabling trp1 cells to grow on medium lacking tryptophan. This powerful selection complements the repertoire of the currently used split-protein sensors and provides a new tool for high-throughput interaction screening. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |