Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy |
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| Authors: | Yin Yandong Wang Peng Yang Xin Xing Li Xun He Chuan Zhao Xin Sheng |
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| Institution: | Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. |
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| Abstract: | Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally. |
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