DNA BREAKAGE CAUSED BY 334-nm ULTRAVIOLET LIGHT IS ENHANCED BY NATURALLY OCCURRING NUCLEIC ACID COMPONENTS AND NUCLEOTIDE COENZYMES |
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| Authors: | J G Peak M J Peak M Maccoss |
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| Institution: | Photobiology Group, Division of Biological and Medical Research, Argonne National Laboratory, Argonne, IL 60439, USA |
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| Abstract: | Abstract— The induction of breaks in DNA in vitro caused by 334-nm UV radíation is enhanced by the following compounds (fluence enhancement factors and concentrations used in parentheses): 4-thiouridine (6.9, 1 m M ), 5-methylamino-2-thiouridine (7.5, 1 m M ), 2-thiouracil (41.0, 1 m M ), riboflavin (14.4.0.1 m M ), and the oxidized (6.8, 1 m M ) and reduced (3.4, 1 m M ) forms of nicotinamide adenine dinucleotide. Anoxia and diazobicyclo(2.2.2)octane reduce the number of DNA breaks caused by 334-nm radiation plus 4-thiouridine by 70 and 76%, respectively. |
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