槲皮素的共振光散射光谱研究

研究了槲皮素(Qu)的共振光散射光谱和吸收光谱，结果表明，pH在3.30～6.50范围内，Qu的共振光散射信号很强且稳定，当pH＞6.50时，共振光散射强度随pH的增大而迅速减小。在pH 4.00的B-R缓冲溶液中，在λ=497 nm处,共振光散射强度在一定浓度范围内与Qu的浓度成线性关系,线性范围为0.0～3.0×10-4 mol·L-1, 相关系数r＞0.999, 检测限为3.1×10-7 mol·L-1。同时运用量子化学计算方法对Qu分子内、分子间氢键进行了计算，理论计算表明: Qu共振光信号增强的原因是Qu分子通过4-4’分子间氢键聚合形成了超分子聚合体, 这一结论和实验得到的光谱数据完全吻合。

Resonance Light Scattering of Quercetin

Resonance light scattering(RLS) and absorption spectra of Quercetin were studied in the present paper. In the solution with pH 3.30 to pH 6.50, the RLS singal was very strong and stable, but decreased sharply with a increase in pH when pH > 6.50. In the B-R buffer solution at pH 4.00, the RLS intensity at 497 nm was maximum and proportional to the concentration of quercetin. The linear range is 0.0-3.0 x 10(-4) mol x L(-1) with the detection limit of 3.1 x 10(-7) mol x L(-1). Quantum chemistry calculation showed that this enhancement of RLS occurred because the neutral molecules of quercetin assembled into super-molecular aggregates by 4-4' hydrogen-bond. This result completely accords with the spectral data from experiments.