Water-soluble cyanine dyes for biological microchip technology |
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Authors: | V. E. Kuznetsova T. A. Luk’yanova V. A. Vasiliskov O. V. Kharitonova A. V. Chudinov A. S. Zasedatelev |
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Affiliation: | (1) V. A. Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, 32 ul. Vavilova, 119991 Moscow, Russian Federation;(2) M. V. Lomonosov Moscow State Academy of Fine Chemical Technology, 86 prosp. Vernadskogo, 119571 Moscow, Russian Federation |
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Abstract: | Novel indodicarbocyanine dyes were obtained and their spectroscopic characteristics were determined. For equal concentrations of the dyes, the relative fluorescence efficiency was measured at the excitation wavelengths λ = 635 and 655 nm and the emission wavelengths λ = 670 and 690 nm, respectively. Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2355–2359, December, 2007. |
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Keywords: | hybridization analysis cyanine dyes oligonucleotides fluorescence |
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