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Water-soluble cyanine dyes for biological microchip technology
Authors:V. E. Kuznetsova  T. A. Luk’yanova  V. A. Vasiliskov  O. V. Kharitonova  A. V. Chudinov  A. S. Zasedatelev
Affiliation:(1) V. A. Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, 32 ul. Vavilova, 119991 Moscow, Russian Federation;(2) M. V. Lomonosov Moscow State Academy of Fine Chemical Technology, 86 prosp. Vernadskogo, 119571 Moscow, Russian Federation
Abstract:Novel indodicarbocyanine dyes were obtained and their spectroscopic characteristics were determined. For equal concentrations of the dyes, the relative fluorescence efficiency was measured at the excitation wavelengths λ = 635 and 655 nm and the emission wavelengths λ = 670 and 690 nm, respectively. Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2355–2359, December, 2007.
Keywords:hybridization analysis  cyanine dyes  oligonucleotides  fluorescence
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