Water-soluble cyanine dyes for biological microchip technology |
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Authors: | V E Kuznetsova T A Luk’yanova V A Vasiliskov O V Kharitonova A V Chudinov A S Zasedatelev |
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Institution: | (1) V. A. Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, 32 ul. Vavilova, 119991 Moscow, Russian Federation;(2) M. V. Lomonosov Moscow State Academy of Fine Chemical Technology, 86 prosp. Vernadskogo, 119571 Moscow, Russian Federation |
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Abstract: | Novel indodicarbocyanine dyes were obtained and their spectroscopic characteristics were determined. For equal concentrations
of the dyes, the relative fluorescence efficiency was measured at the excitation wavelengths λ = 635 and 655 nm and the emission
wavelengths λ = 670 and 690 nm, respectively.
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2355–2359, December, 2007. |
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Keywords: | hybridization analysis cyanine dyes oligonucleotides fluorescence |
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