| 荧光能量转移体系在生化分析中的应用-吖啶橙(AO)-中性红(NR)荧光探针测定DNA研究 |
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| 引用本文： | 陈鸿琪.荧光能量转移体系在生化分析中的应用-吖啶橙(AO)-中性红(NR)荧光探针测定DNA研究[J].化学研究与应用,2000,12(2):164-168. |
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| 作者姓名： | 陈鸿琪 |
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| 作者单位： | 山东临沂师范学院,山东 临沂,276001 |
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| 摘 要： | 本文简述了以荧光能量转移体系测定ＤＮＡ方法的发展过程，对近几年这一领域的新成果作了介绍；同时对吖啶橙（ＡＯ）－中性红ＮＲ为荧光探针测定ＤＮＡ的条件进行分析，证明了该法具有灵敏度高、线性范围宽、检出限低、选择性好、抗干扰能力强等，该法为微量ＤＮＡ定量分析提供了一个新的途径。
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| 关 键 词： | 荧光能量转移 荧光探针 双螺旋结构 DNA测定 |
| 文章编号： | 1004-1656(2000)02-0164-05 |
| 修稿时间： | 1999-10-20 |
APPLICATION OF FLUORECECENCE ENERGY TRANSFER SYSTEM IN BIOCHEMISTRY ANALYSIS-RESEARCH ON TESTING DNA WITH ACRIDINE ORANGE(AO)-NEUTRAL RED (NR) AS A FLUORESCENCE PROBE |
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| CHEN Hong-qi.APPLICATION OF FLUORECECENCE ENERGY TRANSFER SYSTEM IN BIOCHEMISTRY ANALYSIS-RESEARCH ON TESTING DNA WITH ACRIDINE ORANGE(AO)-NEUTRAL RED (NR) AS A FLUORESCENCE PROBE[J].Chemical Research and Application,2000,12(2):164-168. |
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| Authors: | CHEN Hong-qi |
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| Institution: | CHEN Hong-qi
(Shandong Linyi Education College ,Linyi 276001 ,China) |
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| Abstract: | This thesis demonstrates the development of testing DNA with fluorescence energy transfer system and gives an introduction about the new study results of recent years in this field.It also analyses various conditions of testing DNA with acridine orange (AO) neutral red(NR) as a fluorescence probe which proving this method is of high sensitivity,wide linear concentration range,low determination limit,good selectivity and strong ability against interference. |
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| Keywords: | fluorescence energy transfer fluorescence probe double-spiral structure fluorescence quenching Acridine orangy (AO)-neutral red (NR) fluorescence system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
