Preparation and characterization of a new HPLC C18 reversed phase containing thiocarbamate groups |
| |
Authors: | Rosely Barbosa Silva Csar Ricardo Silva Claudio Airoldi Carol H Collins Isabel Cristina SF Jardim |
| |
Institution: | Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13084-971 Campinas, São Paulo, Brazil |
| |
Abstract: | A HPLC stationary phase that possesses an internal thiocarbamate functional group is described. The new C18-thiocarbamate silane was synthesized by the reaction of a trifunctional alkoxysilane with a mercaptan. The silylant agent was bonded to silica (5 μm) and the new stationary phase was then endcapped. Surface characteristics of the packing before and after chemical modification with HMDS and TMCS were determined by different physico-chemical methods, such as elemental analysis and infrared and solid-state 13C and 29Si nuclear magnetic resonance spectroscopies. Chromatographic properties of the C18-thiocarbamate silica were evaluated under reversed phase conditions by separation of four different test mixtures that including compounds from the Engelhardt, Tanaka, and Neue test mixtures. Chromatographic evaluations of the C18-thiocarbamate phase show promising results for the separation of basic analytes. |
| |
Keywords: | Thiocarbamate stationary phase Embedded polar groups HPLC |
本文献已被 ScienceDirect 等数据库收录! |