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| 高效液相色谱紫外检测法测定小麦幼苗中的内源激素 | |
| 引用本文： | 朱智甲,郝振芳,张晓惠. 高效液相色谱紫外检测法测定小麦幼苗中的内源激素[J]. 光谱实验室, 1999, 16(3): 281-283 |
| 作者姓名： | 朱智甲 郝振芳 张晓惠 |
| 作者单位： | 1. 河北师范大学实验中心,石家庄市裕华东路265号,050016 2. 河北师范大学化学系,石家庄市裕华东路265号,050016 3. 河北隆尧师范学校,河北省隆尧县,055350 |
| 摘 要： | 本文从小麦幼苗中提取出三种植物内源激素，并用反相高效液相色谱紫外检测法测定了激素的含量。选择最佳色谱条件及仪器参数，建立了内源激素的高效液相色谱紫外检测法，该法简便，快速，准确度高，重现性好。 |
| 关 键 词： | 内源激素 高效液相色谱法 小麦幼苗 |
| 修稿时间： | 1999-02-13 |
High Performance Liquid Chromatographic(HPLC) Determination of Internal Hormones in Wheat Seedlings |
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| ZHN Zhi-Jia,HAO Zhen-Fang,ZHANG Xiao-Hui. High Performance Liquid Chromatographic(HPLC) Determination of Internal Hormones in Wheat Seedlings[J]. Chinese Journal of Spectroscopy Laboratory, 1999, 16(3): 281-283 | |
| Authors: | ZHN Zhi-Jia HAO Zhen-Fang ZHANG Xiao-Hui |
| Abstract: | This paper describes the application of reversed|phase HPLC to the determination of internal plant hormones including gibberellin(GA3)abscisic acid(ABA) and 3|indoleacetic acid(IAA) in purified extracts of wheat seedlings,and also gives an extraction and purification procedure useful for the analysis of the hormones in plant tissue.A spherisorb C18 column was used and isocratic elution with MeOH|1.8% HAc|H2O(404020) at a flow rate of 2.5mL/min was performed with a UV detector at 254nm. |
| Keywords: | Internal Hormones HPLC Wheat Seedlings. |
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