Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis |
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Authors: | H Nishizawa N Kita S Okimura E Takao Y Abe |
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Affiliation: | Kyoritsu College of Pharmacy, Tokyo, Japan. |
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Abstract: | An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm x 9 cm x 0.05 mm 4-20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights. |
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