Imaging mass spectrometry with silver naeoparticles reveals the distribution of fatty acids in mouse retinal sections |
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Authors: | Takahiro Hayasaka Naoko Goto-Inoue Nobuhiro Zaima Kamlesh Shrivas Yukiyasu Kashiwagi Mari Yamamoto Masami Nakamoto Mitsutoshi Setou |
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Institution: | (1) Department of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama Kanagawa, 226-8501, Japan;(2) Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu Shizuoka, 431-3192, Japan; |
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Abstract: | A new approach to the visualization of fatty acids in mouse liver and retinal samples has been developed using silver nanoparticles
(AgNPs) in nanoparticle-assisted laser desorption/ ionization imaging mass spectrometry (nano-PALDI-IMS) in negative ion mode.
So far, IMS analysis has concentrated on main cell components, such as cell membrane phospholipids and cytoskeletal peptides.
AgNPs modified with alkylcarboxylate and alkylamine were used for nano-PALDI-IMS to identify fatty acids, such as stearic,
oleic, linoleic, arachidonic, and eicosapentaenoic acids, as well as palmitic acid, in mouse liver sections; these fatty acids
are not detected using 2,5-dihydroxybenzoic acid (DHB) as a matrix. The limit of detection for the determination of palmitic
acid was 50 pmol using nano-PALDI-IMS. The nano-PALDI-IMS method is successfully applied to the reconstruction of the ion
images of fatty acids in mouse liver sections. We verified the detection of fatty acids in liver tissue sections of mice by
analyzing standard lipid samples, which showed that fatty acids were from free fatty acids and dissociated fatty acids from
lipids when irradiated with a laser. Additionally, we applied the proposed method to the identification of fatty acids in
mouse retinal tissue sections, which enabled us to learn the six-zonal distribution of fatty acids in different layers of
the retina. We believe that the current approach using AgNPs in nano-PALDI-IMS could lead to a new strategy to analyze basic
biological mechanisms and several diseases through the distribution of fatty acids. |
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