Utilization of an evaporative light scattering detector for high-performance size-exclusion chromatography of galacturonic acid oligomers |
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Authors: | Cameron Randall G Hotchkiss Arland T Kauffman Steven W Grohmann Karel |
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Affiliation: | US Department of Agriculture, Agricultural Research Service, SAA, Citrus and Subtropical Products Laboratory, 600 Ave. S, N.W., Winter Haven, FL 33881, USA. rcameron@citrus.usda.gov |
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Abstract: | A high-performance size-exclusion chromatography-evaporative light scattering detector method was used to separate, detect and quantify galacturonic acid (GA) oligomers. In 40 mM acetic acid GA monomer, dimer and trimer could be separated with baseline resolution but polygalacturonic acid (PGA) precipitated and could not be eluted from the column. An NH4OAc, pH 3.7, buffer was developed as the eluent which separated GA oligomers as well as PGA and pectin without precipitation. Linear calibration curves for mono-, di- and tri-GA were produced with this buffer which could be used to estimate masses of tetra-, penta- and hexa-GA, as well as 19mer and 20mer. |
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