Use of ediap mass spectrometry for determining composition of products obtained in periodate oxidation of sucrose

The possibility has been demonstrated of using EDIAP mass spectrometry for the express determination of the composition of the products obtained in the periodate oxidation of sucrose and its subsequent reduction with NaBH₄. It has been shown that the method permits the amount of oxidized glycol and triol groupings to be determined and can therefore be used successfully to establish the structure of carbohydrates. The method also permits the recording directly in the reaction mixture of the presence of the equilibrium forms produced in the interaction of aldehyde groups with molecules of the solvent or of hydroxylamine. Analysis with the aid of EDIAP mass spectrometry requires 100–200 picomoles, calculated on the initial amount of sucrose. The time of analysis is 5 min. The mass spectra contain only the peaks of quasimolecular ions of the type of [M + Na]⁺.Institute of Analytical Instrument-construction, Leningrad. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 687–695, September–October, 1988.