Model studies on protease-catalysed peptide synthesis using 9-fluorenylmethoxycarbonyl protected amino acid derivatives |
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Authors: | Peter Kuhl Simone Säuberlich Hans-Dieter Jakubke |
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Institution: | (1) Biosciences Division, Department of Biochemistry, Leipzig University, D-O-7010 Leipzig, Federal Republic of Germany |
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Abstract: | Summary
Fmoc-Phe-OMe,Fmoc-Ala-OMe andFmoc-Gly-OH were coupled with H-Leu-NH2 under catalytic action of chymotrypsin, papain and thermolysin, respectively. The influence of different reaction media and several reaction parameters, such as reactants and enzyme concentrations as well as reaction time, on the peptide bond formation was investigated.
Abbreviations: IUPAC-IUB rules for peptides are followed, see (1984) Eur. J. Biochem.138: 9. All amino acids except of Gly are ofL-configuration.DMF=N,N-dimethylformamide,DTE=dithioerythritol,EDTA=ethylenediaminetetraacetic acid, disodium salt,N/C=ratio nucleophile component/carboxyl component,-OMe=methyl ester,TLC=thin layer chromatography. |
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Keywords: | Enzymatic peptide synthesis Fmoc-protected amino acid derivatives Chymotrypsin Papain Thermolysin |
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