Sensitivity improvement in capillary electrophoresis using organo-aqueous separation buffers and thermal lens detection |
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Authors: | Svetlana N Bendrysheva Mikhail A Proskurnin Ute Pyell Werner Faubel |
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Institution: | (1) Chemistry Department, M.V. Lomonosov Moscow State University, Moscow, Russia;(2) Department of Science, Institute of Chemistry, University of Kassel, Kassel, Germany;(3) Department of Chemistry, University of Marburg, Marburg, Germany;(4) Water Technology and Geotechnology Division, Institute of Technical Chemistry, Research Center Karlsruhe, Karlsruhe, Germany |
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Abstract: | It is shown that organo-aqueous separation buffers show much promise when used in capillary electrophoresis separations with
photothermal (thermal lens) detection systems. Acetonitrile–water and methanol–water mixtures were selected, as conventionally
used in capillary electrophoresis. It is shown that, despite more sophisticated experimental conditions (significant heat
outflow from the capillary body) and peak detection, the theoretical ratio of the thermal lens signal for a binary mixture
to the thermal lens signal for an aqueous solution (or the corresponding ratio obtained experimentally under bulk batch conditions)
can be used to predict the sensitivity of thermal lens detection in capillary electrophoresis. The limits of detection for
2-, 3-, and 4-nitrophenols selected as model compounds in 70% v/v acetonitrile separation buffers are 1×10−6 M, 1×10−6 M and 3×10−7 M, respectively, and are therefore decreased by a factor of six compared to thermal lens detection in aqueous separation
buffers. The overall increase in the thermal lens detection sensitivity in a 100% ACN buffer is a factor of 13.
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Keywords: | Photothermal spectroscopy Thermal lens detection Capillary electrophoresis Organo-aqueous solutions Sensitivity Media comparison |
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