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Direct electrochemistry and electrocatalysis of hemoglobin immobilized in TiO2 nanotube films |
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| Authors: | Zheng W Zheng Y F Jin K W Wang N |
| Affiliation: | aLTCS and Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing 100871, China bCenter for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China cDepartment of Physics, The Hong Kong University of Science and Technology, Hong Kong, China |
| Abstract: | Titanium oxide nanotubes (TiO2-NTs) synthesized by the hydrothermal method had been prepared as the co-immobilization matrix to incorporate hemoglobin (Hb) successfully. The nanostructures of TiO2-NTs were investigated by X-ray diffraction and high-resolution electron microscopy. The Hb immobilized in TiO2-NTs had a similar structure to the native of Hb and retained its near-native conformations as characterized by the UV–vis and FT-IR spectroscopy. A couple of quasi-reversible redox peaks with a formal potential of −0.34 V (vs. SCE) in 0.10 M pH 7.0 phosphate buffered saline (PBS) were observed. The amperometric response of the immobilized Hb linearly to H2O2 concentration ranged from 4 μM to 64 μM with a detection limit of 4.637 × 10−6 M and the high stability of the immobilized Hb in TiO2-NTs constituted a promising platform for the development of biosensors. |
| Keywords: | Titanium oxide nanotubes Hemoglobin Electrochemistry Hydrogen peroxide Biosensors |
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