| 新型聚乙烯醇系亲和吸附剂的制备及其在糖蛋白纯化中的应用 (I)基质合成 |
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| 引用本文： | 雷引林,刘坐镇,刘勤凤,宁方红,邬行彦.新型聚乙烯醇系亲和吸附剂的制备及其在糖蛋白纯化中的应用 (I)基质合成[J].离子交换与吸附,2000,16(4):344-350. |
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| 作者姓名： | 雷引林 刘坐镇 刘勤凤 宁方红 邬行彦 |
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| 作者单位： | 生物反应器工程国家重点实验室华东理工大学华震公司,上海 200237 |
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| 基金项目： | 国家自然科学基金，(29706002) |
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| 摘 要： | 以醋酸乙烯酯（ＶＡＣ）和甲基丙烯酸缩水甘油酯（ＧＭＡ）为单体，甲基丙烯酸烯丙酯（ＡＭＡ）为交联剂，在致孔剂正庚烷／乙酸丁酯的存在下，悬浮聚合，制得了一系列大孔共聚物；醇解后得到一种新型聚乙烯醇系亲和层析基质。依据单体的Ｑ、ｅ值，计算了体系的竞聚率，讨论了不同交联度、ＧＭＡ含量、致孔剂用量及配比对基质孔结构和性能的影响。
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| 关 键 词： | 亲和层析 纯化 糖蛋白 聚乙烯吸附剂 |
| 文章编号： | 1001-5493(2000)04-0344-07 |
| 修稿时间： | 1999年11月1日 |
STUDY ON SYNTHESIS OF POLYVINYL ALCOHOL AFFINITY ADSORBENT AND ITS APPLICATION FOR PURIFYING GLYCOPROTEIN ( I ) MATRIX SYNTHESIS |
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| LEI Yinlin LIU Zuozhen LIU Qinfeng NING Fanghong WU Xingyan State Key Lab of Bioreactor Engineering,Huazhen Company,East China University of Science and Technology,Shanghai.STUDY ON SYNTHESIS OF POLYVINYL ALCOHOL AFFINITY ADSORBENT AND ITS APPLICATION FOR PURIFYING GLYCOPROTEIN ( I ) MATRIX SYNTHESIS[J].Ion Exchange and Adsorption,2000,16(4):344-350. |
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| Authors: | LEI Yinlin LIU Zuozhen LIU Qinfeng NING Fanghong WU Xingyan State Key Lab of Bioreactor Engineering Huazhen Company East China University of Science and Technology Shanghai |
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| Institution: | LEI Yinlin LIU Zuozhen LIU Qinfeng NING Fanghong WU Xingyan State Key Lab of Bioreactor Engineering,Huazhen Company,East China University of Science and Technology,Shanghai,200237 |
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| Abstract: | A series of novel macroporous polyvinyl alcohol copolymers (VGA) were synthesized using Vinyl acetate (VAC) and Glycidyl methacrylate (GMA) as monomers and Allyl methacrylate (AMA) as crosslinking agent by suspension copolymerization under the existence of mixed porogenic agent of n-heptane and butyl acetate. The effects of the crosslinking degree, the amount of GMA and porogenic agent, and the proportion of porogenic agent on copolymer performance and pore structure were studied. The alcoholysis products of copolymers can be used to affinity chromatography as matrices because of its strong hydrophilicity. From the Q and e values determined by former investigaters, the reactive ratios of the ternary system of VAC-GMA-AMA were calculated according to Alfrey-Price's Q-e equation. The calculated results indicated the copolymerization of system was possible. |
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| Keywords: | Vinyl acetate Glycidyl methacrylate Allyl methacrylate Affinity Chromatography |
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