Determination of phenazopyridine in human plasma by high performance liquid chromatography |
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Authors: | D Farin G Piva R Kitzes-Cohen |
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Institution: | (1) Clinical Pharmacology and Infectious Diseases Unit, Carmel Medical Center, The Rappaport Faculty of Medicine, Technion, Haifa, Israel |
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Abstract: | Summary A simple, low-cost, sensitive and selective HPLC method was developed for the determination of phenazopyridine in human plasma.
The method employs UV detection of phenazopyridine and of the internal Standard at 2 different wavelengths. Calibration curves
were linear over a large dynamic range, i.e., within 0.05–10.0 μg mL−1 with limit of quantification of 0.05 μg mL−1, and a limit of detection of 0.01 μg mL−1. |
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Keywords: | Column liquid chromatography Phenazopyridine Human plasma |
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