ICAM-1/LFA-1 interaction contributes to the induction of endothelial cell-cell separation: implication for enhanced leukocyte diapedesis |
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Authors: | Hyun Wee Hyun-Mee Oh Jae-Hyeok Jo and Chang-Duk Jun |
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Institution: | Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, Research Center for Biomolecular Nanotechnology, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Korea. |
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Abstract: | The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-α induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules-1 (ICAM-1)/leukocyte function-associated antigen-1 interaction, as demonstrated by the following: (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration. |
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Keywords: | cell movement endothelial cells intercellular adhesion molecule-1 leukocytes lymphocyte function-associated antigen-1 |
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