| 大豆中脲酶活力的玻璃电极法测定 |
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| 引用本文： | 汪乃兴 张建国. 大豆中脲酶活力的玻璃电极法测定[J]. 分析化学, 1994, 22(5): 478-481 |
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| 作者姓名： | 汪乃兴 张建国 |
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| 作者单位： | 复旦大学化学系!上海200437(汪乃兴，王彦刚，赵滨，邵海燕)，济宁教育学院生化科!济宁272137(张建国) |
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| 摘 要： | 本文用玻璃电极电位法研究了大豆及脲酶制剂中脲酶活力的测定和酶的反应动力学，测定了米氏常数ＫＭ以及酶催化反应最大速度ｒｍ，方法快捷简便。
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| 关 键 词： | 大豆 脲酶 活力 测定 玻璃电极法 |
Determination of Urease Activity in Soybeen by Glass Electrode Potentiometry |
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| Wang Naixing,Wang Yangang,Zhao Bin,Shao Haiyan. Determination of Urease Activity in Soybeen by Glass Electrode Potentiometry[J]. Chinese Journal of Analytical Chemistry, 1994, 22(5): 478-481 |
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| Authors: | Wang Naixing Wang Yangang Zhao Bin Shao Haiyan |
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| Abstract: | The determination of urease activity in soybeen and urease manufactures, and kinetics of enzyme catalytic reaction have been investigated by glass electrode potentiometry. This method provides a rapid and simple procedure for the direct determination of urease activity and the Michaelis-constant K_M and the maximum velocity r_m for enzyme catalytic reaction. |
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| Keywords: | Urease Activity determination Potentiometry |
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