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Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation |
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| Authors: | Christopher M Earhart Robert J Wilson Nader Pourmand |
| Institution: | a Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA b Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA c Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, USA |
| Abstract: | A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (∼5000/mm2) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labeled MACS nanoparticles. Capture efficiencies ranging from 28% to 37% and elution efficiencies greater than 73% were measured for a single pass through the sifter. |
| Keywords: | Magnetic sifter High-gradient magnetic separation Magnetic nanoparticle Cell separation CD138 Microfluidic |
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