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Characterization of magnetite nanoparticles for SQUID-relaxometry and magnetic needle biopsy |
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| Authors: | Natalie L. Adolphi Dale L. Huber Howard C. Bryant Danielle L. Fegan Todd C. Monson Helen J. Hathaway Richard S. Larson |
| Affiliation: | a Senior Scientific, LLC, 11109 Country Club NE, Albuquerque, New Mexico 87111, USA b Sandia National Laboratories, P.O. Box 5800, Albuquerque, New Mexico 87185, USA c Department of Pathology, University of New Mexico, Cancer Research and Treatment Center, Albuquerque, New Mexico 87131, USA d Department of Cell Biology and Physiology, University of New Mexico, Albuquerque, New Mexico 87131, USA e Chemicell, GmbH, Eresburgstrasse 22−23, 12103 Berlin, Germany |
| Abstract: | Magnetite nanoparticles (Chemicell SiMAG-TCL) were characterized by SQUID-relaxometry, susceptometry, and TEM. The magnetization detected by SQUID-relaxometry was 0.33% of that detected by susceptometry, indicating that the sensitivity of SQUID-relaxometry could be significantly increased through improved control of nanoparticle size. The relaxometry data were analyzed by the moment superposition model (MSM) to determine the distribution of nanoparticle moments. Analysis of the binding of CD34-conjugated nanoparticles to U937 leukemia cells revealed 60,000 nanoparticles per cell, which were collected from whole blood using a prototype magnetic biopsy needle, with a capture efficiency of >65% from a 750 μl sample volume in 1 min. |
| Keywords: | Magnetite Nanoparticle Magnetorelaxometry SQUID detection Susceptometry Antibody-conjugation |
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