Determination of pyrethroid metabolites in human urine by capillary gas chromatography-mass spectrometry |
| |
| Authors: | K. -H. Kühn G. Leng K. A. Bucholski L. Dunemann H. Idel |
| |
| Affiliation: | (1) Institute of Hygiene, University of Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany;(2) Department of Analytical Chemistry, Medical Institute of Environmental Hygiene at the University of Düsseldorf, Auf'm Hennekamp 50, 40225 Düsseldorf, Germany |
| |
| Abstract: | Summary An analytical method for the simultaneous determination of the pyrethroid metabolites cis and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid, cis 3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane carboxylic acid, 3-phenoxybenzoic acid and 4-fluoro-3-phenoxybenzoic acid in human urine samples is described. The urine is subjected to acid-induced hydrolysis followed by exhaustive solvent extraction, covering both conjugated and free acids, followed by a common derivatisation step yielding the corresponding methyl esters. Quantitation was by diastereomeric, capillary gas chromatography-mass spectrometry. It appears that 4-fluoro-3-phenoxybenzoic acid is a characteristic urinary marker for cyfluthrin exposure. The limits of determination are 0.5–1.0  g L–1 urine depending on the metabolites concerned. The applicability of the method was tested on urine samples from pest control operators exposed occupationally to cypermethrin and cyfluthrin. |
| |
| Keywords: | Gas chromatography-mass spectrometry Pyrethroid metabolites in urine Urinary marker 4-fluoro-3-phenoxybenzoic acid Cypermethrin and cyfluthrin |
| 本文献已被 SpringerLink 等数据库收录！ |
|
