Potentiality of partial least-squares multivariate calibration in the spectrophotometric analysis of binary mixtures of purine bases |
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Authors: | H. Khajehsharifi and Z. Eskandari |
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Affiliation: | (1) Department of Chemistry, Yasouj University, Yasouj, Iran |
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Abstract: | Abstract A partial least-squares (PLS) modeling was developed for the simultaneous spectrophotometric determination of adenine (AD) and guanine (GU). The determination of these analytes is pharmacologically necessary. Multivariate calibration is used because of spectral overlapping. The calibration set contained AD and GU in the concentration range of 1.4–20.3 and 1.5–25.7 μg cm−3, respectively. The absorption spectra were recorded from 200 to 300 nm. The predicted residual error sum-of-squares for AD and GU was 0.0500 and 0.4000 for number of principal components 3 and 2, respectively. The root mean square error of prediction for AD and GU was 0.0913 and 0.2582, respectively. The limits of detection were 0.02 and 0.03 μg cm−3 for AD and GU, respectively. The proposed method allows the simultaneous determination of AD and GU in spiked real matrixes of human urine, serum, and plasma. |
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