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Amperometric nitrite biosensor based on a gold electrode modified with cytochrome c on Nafion and Cu-Mg-Al layered double hydroxides |
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| Authors: | Huanshun Yin Yunlei Zhou Tao Liu Lin Cui Shiyun Ai Yanyan Qiu Lusheng Zhu |
| Institution: | 1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, Shandong, China 2. College of Resources and Environment, Shandong Agricultural University, Taian, 271018, Shandong, China 3. College of Life Science, Beijing Normal University, 100875, Beijing, China |
| Abstract: | An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transform infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 μM. The detection limit is 2?×?10?7 M (S/N?=?3). The biosensor was successfully applied to the determination of nitrite in food samples. |
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