污水处理厂及受纳水体样品的三维荧光光谱解析

采用三维荧光光谱表征了污水厂各处理单元及受纳水体上下游的样品，应用平行因子分析方法获得了样品中各主成分的激发发射光谱图及荧光强度得分矩阵。结果表明，类蛋白质和类富里酸物质是污水厂和受纳水体样品的主要荧光组分。污水厂进水样品的类蛋白质荧光较强，后续各处理单元样品的荧光强度显著下降。受纳水体上游样品的类蛋白质荧光较弱，经过城区各取样点的类蛋白质荧光显著增加，而污水厂排放口上游荧光强度则显著高于下游。类蛋白质荧光强度得分可与样品COD值建立相关曲线，污水厂与受纳水体样品的相关系数分别为0.930和0.913，类蛋白质荧光可以反映样品点的有机污染程度。该研究为污水处理厂的运行及其对受纳水体影响提供了新的思路和方法。

Analysis of Samples from Wastewater Treatment Plant and Receiving Waters Using EEM Fluorescence Spectroscopy

The samples from several treatment units in wastewater treatment plant (WWTP) and different sample sites of receiving waters were characterized using excitation-emission matrix (EEM) fluorescence spectroscopy. After analyzed by parallel factor analysis (PARAFAC) method, the EEM fluorescence spectra of the principal components and fluorescence intensity scores matrix were acquired. Results showed that protein-like and fulvic-like substances were the main components of the samples from the WWTP and the receiving water body. The intensity of protein-like fluorescence in the influent samples was rather strong, and those of the following samples decreased significantly. The protein-like fluorescence intensity of the samples was relatively weak from the upstream of receiving water body, while those of the samples increased significantly from the water body near the urban area. The protein-like fluorescence intensity of upstream samples of the WWTP disposal outlet was stronger than that of downstream in the receiving waters. The fluorescence intensity scores of protein-like substance could be correlated with the COD concentration of the samples and the correlation curves were established. The correlation coefficient of the WWTP samples was 0.930 and that of receiving water body samples was 0.913. The protein-like fluorescence could be used to evaluate the organic pollution of the samples. This study will provide a new method to investigate the operation status of WWTP and corresponding effect on the receiving water body.