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Antioxidant Properties and Phenolic Composition of Sideritis Species
Authors:Z. Tunalier  M. Kosar  N. Ozturk  K. H. C. Baser  H. Duman  N. Kirimer
Affiliation:(1) Faculty of Pharmacy, Department of Pharmacognosy, Anadolu University, 26470 Eskisehir, Turkey;(2) Faculty of Science and Letters, Department of Biology, Gazi University, 06500, Ankara
Abstract:The antioxidant properties and phenolic composition of 27 Sideritis species were studied. Plant samples were extracted with petroleum ether using a Soxhlet apparatus. The defatted plant materials were extracted with 70% methanol. Antioxidant activities of the extracts were measured using Fe+2 induced linoleic acid peroxidation, as indicated by thiobarbituric acid reactive substance (TBARS) production. Free radical scavenging activities were determined based on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Results were compared with standard BHT. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard, and phenolic components were quantified by HPLC-DAD.
Keywords:Sideritis  antioxidant  HPLC  free radical scavenging activity  lipid peroxidation  composition
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