荧光光谱法研究碳纳米管与牛血清白蛋白间相互作用

作为一类新型的纳米材料,碳纳米管(CNTs)所具有的独特结构、强细胞穿透能力和低免疫原性等特性引发了生物医学领域的广泛关注,并有望作为一类新型的药物和基因转运载体。因此,研究碳纳米管与生物大分子间的相互作用对其在体内的进一步应用具有极其重要的意义。本文运用荧光猝灭法、同步荧光法以及红边激发荧光位移法(REES法)研究了生理条件下碳纳米管与牛血清白蛋白(BSA)间的相互作用。结果表明,碳纳米管可引起BSA内源性荧光的静态猝灭,但同步荧光和REES的研究结果表明碳纳米管对BSA的构象基本没有影响。同时,通过对碳纳米管与BSA相互作用的机制的探讨,我们推测碳纳米管主要通过非特异性吸附的方式与BSA相互作用。

Fluorescence study on the interactions between carbon nanotubes and bovine serum albumin

As a new family of nanomaterials, carbon nanotubes (CNTs) exhibit unique properties such as their capacity to penetrate into the cells and low immunogenicity, which have attracted increasing attention in biomedical field and make their application in drug and gene transfer systems being possible. So getting more information about the interaction of CNTs with biomacromolecules is crucial for further investigation of CNTs in therapeutic applications. The interaction between CNTs and BSA under physiological condition was investigated by fluorescence quenching, synchronous fluorescence and red edge excitation shift (REES)method. The results showed that CNTs could significantly decrease the fluorescence intensity of BSA. While the results of synchronous fluorescence and REES indicated that CNTs almost had no influence on the conformation of BSA. So the authors concluded that the mechanism of interaction between CNTs and BSA was non-specific adsorption.