罗丹明B与基因共负载纳米粒子的制备与性能

药物/基因共负载的智能微载体的制备是肿瘤多元复合治疗的关键科学问题。本文以疏水性荧光染料罗丹明B为模型药物，采用聚乙烯亚胺－接枝－胆固醇两亲聚合物作为药物载体，成功制备了罗丹明与基因共负载的超分子组装体。通过组装条件的调控，获得了尺寸为150 nm、表面电位33 mV的球形纳米粒子，并依然具有很好的DNA缔合特性。细胞培养结果表明：表面正电荷的罗丹明与基因共负载纳米粒子很容易被细胞内吞，并能有效转染细胞，为高效安全的药物/基因共负载微载体的制备提供了切实可行的途径。 关键词 非病毒基因载体；超分子组装；药物/基因共负载

Construction of Rhodamine B and Gene Co-delivery Nanoparticles

The preparation of drug and gene co-delivery nanoparticles was the key scientific problem in tumor multi-combined therapy. In this research, the hydrophobic fluorescent dye rhodamine B was chosen as a model drug. PEI-g-cholesterol amphiphilic polymer was synthesized as drug carrier. By selecting proper conditions, rhodamine B and gene co-delivery nanoparticles were successfully constructed via supramolecular assembly. The co-delivery particles with diameter about 150 nm and ξ-potential about 33 mV could effectively condense DNA. The in-vitro cell culture experiments indicated that the positive co-delivery nanoparticles was easily uptaked by HEK293 cells and showed effective transfection efficiency.