非标记液晶型免疫传感器检测赭曲霉素A

研制了一种基于液晶取向改变的非标记液晶型免疫传感器,并用于检测赭曲霉素A(0TA).采用戊二醛交联法将OTA同定在由自组装膜修饰的玻璃肇底表面.自组装膜能诱导液晶分子垂直排列,而连接OTA抗体后则扰乱了液晶分子取向的有序排列,导致液晶分子在化学敏感膜表面的取向发生变化,使光学信号的亮度及色彩发生变化,以此实现对OTA的...

Detection of Ochratoxin A by Label-Free Liquid Crystal Immunosensor

A novel liquid crystal immunosensor based on the indirect competitive assay format was developed for the detection of ochratoxin A (OTA). In this method, the OTA was tethered onto the self-assembled film surface on a glass slide via a cross-linker glutaradehyde. The specific binding event of anti-OTA antibody and ochratoxin A induced the homeotropic-to-tilted transition of LCs, resulting in the corresponding changes of opitical images under the crossed polarized light. The LC-based imaging method has a good signal-to-noise ratio and a clear distinction between positive and negative results. When the concentration of analyte OTA exceeds a critical value (0.01 ng/mL) , the opitical images gives a very sharp response. The opitical images changes with the concentration of OTA in a dynamic range of 0.01–10 ng/mL .The fabricated sensor is show to exhibit high sensitivity, desirable selectivity, label free and ease in operation。