Kinetic study of the performance of third-generation biosensors |
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| Institution: | 1. Department of Chemistry, Lomonosov Moscow State University, 1–3 Leninskiye Gory, Moscow 119991, Russia;2. Department of Physical Chemistry, Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, 31 Leninsky Prospect, Moscow 119991, Russia |
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| Abstract: | A kinetic study of the performance of third-generation biosensors for glucose based on glucose oxidase immobilized on a microporous matrix of the conducting polymer poly(pyrrole) is presented. The mechanism of the enzymatically catalyzed oxidation of glucose will be different in this type of biosensor as the natural electron acceptor oxygen is replaced by the conducting polymer. Different kinetic parameters are found for the immobilized glucose oxidase than for the enzyme in solution. Mediation by the conducting polymer is found to be very effective and no significant electron transfer to oxygen is observed. In addition to substrate transport limitation in the microporous matrix, the enzymatic reaction in the biosensors is limited by the applied potential. |
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