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Ordered arrays of myoglobin adsorbed on the surfactant modified surface by scanning tunneling microscopy
Institution:1. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193,China;2. Institute of Agro-products Processing Science and Technology, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China;3. Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China;4. Key Laboratory of Agricultural Product Processing and Quality Control of Specialty(Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China;1. School of Food and Bioloical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China;2. Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, Zhenjiang, Jiangsu 212013, China;3. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China;4. Department of Agricultural, Food and Nutritional Science (AFNS), 4-10 Ag/For Centre, University of Alberta, Edmonton T6G 2P5, Alberta, Canada;1. Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 117543 Singapore, Singapore;2. National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China;3. School of Agriculture and Food Sciences, The University of Queensland, Brisbane 4072, Australia;1. Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, College of Food Science and Engineering, Hainan University, Haikou 570228, China;2. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China;3. Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China;4. Institute of Food Science and Engineering, Hezhou University, Hezhou 542899, China;1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China;2. National R & D Branch Center for Conventional Freshwater Fish Processing, Wuhan, Hubei 430070, PR China;3. Department of Chemistry, Wuhan University, Wuhan 430072, PR China;4. College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, PR China;5. School of Human and Social Sciences, University of West London, London, UK;1. Department of Biology, Shahrekord University, P.O. Box: 115, Shahrekord, Iran;2. Central Laboratory, Shahrekord University, Shahrekord, Iran
Abstract:Myoglobin molecules were deposited on a surfactant sodium dodecyl sulfate modified HOPG surface and imaged in air with a high resolution scanning tunneling microscope (STM) for the first time. STM images exhibit not only ordered arrays of the surfactant molecules but also regular two-dimensional arrays of myoglobin molecules. From STM images, the myoglobin molecule can be described as an ellipsoid-shaped pattern for the tertiary structure. In this study the dimensions of a myoglobin molecule were determined approximately as 43.0 × 36.2 × 8 Å3, which are in good agreement with the known data from X-ray analysis, except for the height of a molecule which cannot be estimated from STM.
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