A kinetic study of soluble glucose oxidase using a rotating-disc electrode |
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| Affiliation: | 1. Department of Chemistry “Ugo Schiff” & CSGI, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy;2. Pavia H2Lab, Department of Chemistry & CSGI, c, Via Taramelli 16, Pavia 27100, Italy;3. Department of Physical and Colloid Chemistry, Ivan Franko National University of Lviv, Kyryla & Mefodiya 6, Lviv 79005, Ukraine;4. Institute of Chemistry, Lviv Polytechnic National University, Bandery 12, Lviv 79013, Ukraine;1. College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China;2. College of Biological, Chemical Science and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China;3. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, China;1. Department of Tumor Cell Biology, St. Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA;2. Department of Chemistry, Rhodes College, 2000 N. Parkway, Memphis, TN 38112, USA;3. Center for Integrated Protein Science at the Department of Chemistry and Institute for Advanced Study, Technische Universität München, Lichtenbergstr. 4, 85748 Garching, Germany |
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| Abstract: | In order to determine the kinetic parameters of glucose oxidation catalysed by the enzyme glucose oxidase (GO) the initial velocity of hydrogen peroxide formation was measured using a rotating disc electrode (RDE). The major advantage of this method is the possibility of continuous measurement of the increase in hydrogen peroxide concentration. This means that the real initial reaction rate V0 can be determined, which is required for constructing a double-reciprocal plot. Several combinations of substrate concentrations (glucose and oxygen) were used. The method, in which a platinum black RDE was used, appeared to be very useful. Product inhibition experiments showed that the ping-pong mechanism is valid for GO. The three kinetic parameters of this mechanism were determined by initial velocity experiments. |
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