间充质干细胞扩增载体材料

间充质干细胞(MSCs)具有高度自我更新能力、多分化潜能、体外易分离和培养的特性,是细胞治疗和组织工程重要的种子细胞来源,但如何大规模地获得具有可再生活性的MSCs一直是限制其临床应用的关键因素,近几年发展起来的贴壁动物细胞动态培养技术为MSCs的大规模体外扩增提供了一条重要的途径。本综述结合动物细胞扩增载体的发展现状,主要介绍了用于间充质干细胞三维动态培养的明胶载体、海藻酸盐载体、壳聚糖载体和其他多糖载体等常规载体及其表面修饰和改性方法,并进一步介绍了以非酶解途径回收扩 增细胞的新型干细胞载体的研究进展。随着新型载体材料的涌现以及人们对干细胞生长和扩增特点的了解,采用三维动态培养技术安全而有效地大规模体外扩增MSCs的必要性将得到进一步的确认。

Carrier Materials of Mesenchymal Stem Cells Expansion

Mesenchymal stem cell(MSC) is an important cell source of cell therapy and tissue engineering because of its characteristics of self-renewal,multi-differentiation potential,easily isolated and cultured in vitro.Expansion of MSCs in vitro is a necessary step in clinical application of MSCs since it is impossible to get enough MSCs directly from donors.Namely,how to culture MSCs in large-scale is the key factor to limit its application.The methodology of 3-D dynamic culture of anchorage-dependent cells provides an important way for expansion of MSCs in vitro.This review intends to overview the current progress in the MSCs expansion field and discusses the main events that have occurred along the way.Gelatin,alginate,chitosan and some other polysaccharide carrier materials used for 3-D culture of mammalian cells and MSCs are summarized and discussed.The surface modification methodologies of the microcarriers are also presented.Furthermore,some new carrier materials used for stem cells expansion are introduced.The technical advances together with the ever increasing knowledge and experience in the field of carrier materials preparation and MSCs proliferation/expansion characteristics will lead to the realization of the full potential of 3-D dynamic MSCs culture in the future.