High concentration trans form unsaturated lipids detected in a HeLa cell by Raman microspectroscopy |
| |
Authors: | Chikao Onogi Michiyo Motoyama Hiro‐o Hamaguchi |
| |
Abstract: | High concentration trans form unsaturated lipids have been found in a HeLa cell by Raman microspectroscopy. Two CC stretch bands are observed simultaneously at 1669 cm−1 (trans form) and at 1656 cm−1 (cis form) in a Raman spectrum obtained from a small area (1 µm in diameter) in a HeLa cell. The intensity ratio 1669/1656 indicates that the concentration of the trans form is as high as that of the cis. It is demonstrated that Raman microspectroscopy provides a powerful and unique means for in situ and noninvasive structural characterization of unsaturated lipids in a living cell. Copyright © 2008 John Wiley & Sons, Ltd. |
| |
Keywords: | trans unsaturated lipids HeLa cell confocal Raman microspectroscopy oxidative stress cancer |
|