Rapid analysis for cadmium metallothionein complexes by HPLC using microparticulate stationary phases |
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| Authors: | Hubert Chassaigne R Łobiński |
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| Institution: | Laboratoire de Chimie Bio-inorganique et Environnement, CNRS EP132, Hélioparc, 2, avenue Pr. Angot, F-64?000 Pau, France e-mail: Ryszard.Lobinski@univ-pau.fr, FR
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| Abstract: | Different columns with microparticulate (1.5 and 2 μm) stationary phases were investigated for the analysis of the polymorphism of mammalian metallothionein (MT) by reversed-phase HPLC. When a non-porous 1.5 μm stationary phase was used, the duration of the chromatographic run was reduced 10-fold (in comparison with the conventional 5 μm packing) without any loss in resolution. The method was applied to the analysis of MT-1 and MT-2 preparations from rabbit liver. |
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