Bilayer disk capillary electrophoresis: a novel method to study drug partitioning into membranes |
| |
Authors: | Boija Elisabet Lundquist Anna Nilsson Mikael Edwards Katarina Isaksson Roland Johansson Gunnar |
| |
Institution: | Department of Biochemistry and Organic Chemistry, Biomedical Center, Uppsala University, Uppsala, Sweden. |
| |
Abstract: | CE in the presence of lipid bilayer disks was introduced as a new approach in membrane partitioning studies. The disks were used as a pseudostationary phase in the partial-filling mode of CE and the partitioning of cationic drugs was determined. The migration times of the analytes increased linearly with the lipid amount in the system. An appropriate algorithm for the calculation of a partition coefficient is presented. In the disk-shaped bilayers, which have excellent stability and shelf life, all of the lipids are readily available for interaction and the disks can be used as realistic cell membrane models. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|