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pH Dependent Thermal Stabilization of DNA by Glcβ(1→3)GlcNAcβ1→STol |
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| Authors: | Chun‐Cheng Lin Sue‐Mei Hung Kuo‐Cheng Lu Chien‐Chung Cheng |
| Abstract: | A disaccharide, Glcβ(1→3)GlcNAcβ1→STol (GGS, 1 ), was synthesized and demonstrated to stabilize ct‐DNA during the denaturing process. GGS at 50 μM shifted Tm of ct‐DNA by 23 °C and the behavior was pH dependent. Poly(dA‐dT)2 was found to be the preferable type of DNA for GGS stabilization by circular dichroism spectroscopy study. |
| Keywords: | DNA‐carbohydrate interaction Hydrogen‐bond pH dependent Thermal stabilization |