Solid-phase synthesis of O-glycosylated Nalpha-Fmoc amino acids and analysis by high-resolution magic angle spinning NMR |
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Authors: | Yao Nian-Huan He Wen-Yi Lam Kit S Liu Gang |
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Affiliation: | Chinese Academy of Medical Sciences and Peking Union College of Medicine, Institute of Materia Medica, 1 Xian Nong Tan Street, Beijing 100050, P. R. China. |
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Abstract: | Direct O-glycosylation of amino acids bound to TentaGel resin with a number of glycosyl trichloroacetimidate donors results in high yields. The glycosylation reaction can be easily monitored by analyzing the bead-bound amino acids with high-resolution magic angle spinning (HR-MAS) NMR. These studies pave a new way for the construction of "one-bead one-compound" O-glycopeptide libraries with standard amino acid building blocks and appropriate glycosyl trichloroacetimidate donors. |
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