Molecular weight and monosaccharide composition of Astragalus polysaccharides |
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Authors: | Xu Du-Juan Xia Quan Wang Jia-Jia Wang Pei-Pei |
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Affiliation: | Department of Pharmacy, First Affiliated Hospital of Anhui Medical University, Hefei, 230022. PR China. xudujuan6365@yahoo.com.cn |
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Abstract: | Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH(2) column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively. |
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